FREMGANGSMAADE TIL RENSNING OG FRAKTIONERING AF VANDOPLOESTE, FORTRINSVIS ENZYMATISK AKTIVE PROTEINER

Proteins can be enriched and/or purified by use of polyethylene imines (I) as precipitants. Pref., I have mol. wt 600-100,000. Protein pptn. occurs at low salt concns. and pH 5.0-9.0. At high salt concn. proteins remain in soln., and other contaminants, e.g. nucleic acids are selectively pptd.

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Bibliographische Detailangaben
Hauptverfasser: BERGMEYER HANS ULRICH, THUM WALDEMAR, NAEHER GOTTHILF, WEIMANN GUENTER
Format: Patent
Sprache:dan
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Zusammenfassung:Proteins can be enriched and/or purified by use of polyethylene imines (I) as precipitants. Pref., I have mol. wt 600-100,000. Protein pptn. occurs at low salt concns. and pH 5.0-9.0. At high salt concn. proteins remain in soln., and other contaminants, e.g. nucleic acids are selectively pptd.