Forbedret fremgangsmåde til dyrkning af pattedyrceller in vitro

The invention relates to a method for reduction of glucose consumption and/or lactate production during cultivation of animal cells, characterized in that cultivation is performed in the presence of a bi- or tricarbonic acid or a salt thereof at a concentration of about 1 to 50 mmol/l, whereby in th...

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Bibliographische Detailangaben
Hauptverfasser:	EBERHARDT, HORST, SZPERALSKI, BERTHOLD, BEHRENDT, ULRICH
Format:	Patent
Sprache:	dan
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to a method for reduction of glucose consumption and/or lactate production during cultivation of animal cells, characterized in that cultivation is performed in the presence of a bi- or tricarbonic acid or a salt thereof at a concentration of about 1 to 50 mmol/l, whereby in the case where said di- or tricarbonic acid is citric acid or citrate this amount of said citric acid or citrate is not bound in chelate complex with iron or another transition metal ion.