FREMGANGSMADE TIL FREMSTILLING AF KALVEDIARREVIRUSVACCINE

1276218 Calf diarrhoea vaccine C A MEBUS and M J TWIEHAUS 26 Nov 1970 [28 Nov 1969] 56205/70 Heading A5B Calf diarrhoea virus is attenuated by passaging in one of the following tissue cultures: primary fetal bovine kidney cells, primary fetal bovine lung cells, primary fetal bovine thyroid cells, primary porcine kidney cells, primary fetal bovine choroid plexes cells, primary lamb kidney cells, bovine kidney cell line, embryonic bovine trachea cell line, baby hamster kidney clone cell line, green monkey kidney cell line, pig kidney cell line, swine testicle cell line and Henle's intestine (human embryo) cell line. In typical Example I, the cells are grown on Earle's lactalbumin hydrolyzate and calf serum. Calf passage fecal filtrate is added to the culture which is incubated in a growth medium comprising Earle's balanced salt solution, lactalbumin, yeast, penicillin and streptomycin.