Fremgangsmåder til quenching i opløsning af fluorescens-mærkede oligonnukleotidprober

Method for controlling the light emission of a single-stranded oligonucleotide covalently labelled with a light-emitting label comprises adding a DNA-binding cpd. to a soln. of the labelled oligonucleotide, where the DNA-binding cpd. is capable of interacting with the label to modify its light emiss...

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Bibliographische Detailangaben
Hauptverfasser:	FISHER, MARY ELLEN, WATSON, ROBERT MALCOLM
Format:	Patent
Sprache:	dan
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	Method for controlling the light emission of a single-stranded oligonucleotide covalently labelled with a light-emitting label comprises adding a DNA-binding cpd. to a soln. of the labelled oligonucleotide, where the DNA-binding cpd. is capable of interacting with the label to modify its light emission. Also claimed are: (1) a method for detecting cleavage of a labelled oligonucleotide and (2) a method for detecting a target nucleic acid sequence in a sample, comprising measuring the light emission of a PCR mixt.