Fremgangsmåde til kontinuerlig tilberedning af et sterilt næringsmedie

PCT No. PCT/EP93/01118 Sec. 371 Date Jul. 11, 1994 Sec. 102(e) Date Jul. 11, 1994 PCT Filed May 7, 1993 PCT Pub. No. WO93/23525 PCT Pub. Date Nov. 25, 1993.A method for continuously preparing a sterile culture medium comprises combining separate unsterilized fluid components in separate streams into...

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Hauptverfasser: KUCHENBECKER, RALF, ROSENTHAL, WERNER, MANNWEILER, KLAUS
Format: Patent
Sprache:dan
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Zusammenfassung:PCT No. PCT/EP93/01118 Sec. 371 Date Jul. 11, 1994 Sec. 102(e) Date Jul. 11, 1994 PCT Filed May 7, 1993 PCT Pub. No. WO93/23525 PCT Pub. Date Nov. 25, 1993.A method for continuously preparing a sterile culture medium comprises combining separate unsterilized fluid components in separate streams into a single mainstream. The mainstream is applied to a transverse flow filtration module having a membrane which separates the mainstream into a permeate and a retentate. The permeate is fed to a bioreactor and the retentate is diverted to a centrifuge to precipitate contaminants. The clarified retentate is returned into the mainstream upstream of the transverse flow filtration module.