Testsatz und Methode für eine Bestimmung durch kompetitive spezifische Bindung

One or more specific binding ligands can be detected with a competitive immunoassay which utilizes a water-soluble conjugate of ligand and reporter enzyme for each target ligand. Target ligand is allowed to compete for a first receptor with the conjugate. Uncomplexed conjugate is then contacted with...

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Bibliographische Detailangaben
Hauptverfasser: SNYDER, BRIAN A, CONTESTABLE, PAUL B
Format: Patent
Sprache:ger
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Beschreibung
Zusammenfassung:One or more specific binding ligands can be detected with a competitive immunoassay which utilizes a water-soluble conjugate of ligand and reporter enzyme for each target ligand. Target ligand is allowed to compete for a first receptor with the conjugate. Uncomplexed conjugate is then contacted with an immobilized second receptor to form a reaction product which can be used to generate a detectable signal. This assay provides a direct correlation of the generated signal to the one or more target ligands.