Verfahren für die Detektion und die Identifizierung von viralen und subviralen Pathogenen

Based on the immobilisation of the pathogen by using antibodies attached to the inner surface of the cups of a microtitration plate, with subsequent synthesis, on the same plate, of the complementary DNA with a fragment of the genom of viral or subviral pathogens and subsequent enzymatic amplificati...

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Bibliographische Detailangaben
Hauptverfasser:	TORRES PASCUAL, VICENTE, DE BLAS BEORLEGUI, CARMEN, BORJA TOME, MA JOSE, BARBOSA NOLASCO, NUNO, PONZ ASCASO, FERNANDO
Format:	Patent
Sprache:	ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	Based on the immobilisation of the pathogen by using antibodies attached to the inner surface of the cups of a microtitration plate, with subsequent synthesis, on the same plate, of the complementary DNA with a fragment of the genom of viral or subviral pathogens and subsequent enzymatic amplification on the same plate, then proceeding to quantify the products of amplification by using spectrophotometric techniques, also carried out on the same plate, and finally, the identification of the products of amplification by means of electrophoretic techniques.