Vorrichtung und Verfahren zur räumlich begrenzten Reaktion und Detektion

It is known to carry out PCR amplification and detection using a reaction vessel in which solid particles are fixed and liquid sample and reagents are moved past these particles. Described herein is a device (10, 30) and method for carrying out such reactions. Solids (for example, beads (16)) are us...

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Hauptverfasser:	CUMMINS, THOMAS JOSEPH, EASTMAN KODAK COMPAN, ROCHESTER, NEW YORK 14650-2201, US, OAKES, FRED TERRY, EASTMAN KODAK COMPANY, ROCHESTER, NEW YORK 14650-2201, US, GREEN, NANCY FAME, EASTMAN KODAK COMPANY, ROCHESTER, NEW YORK 14650-2201, US
Format:	Patent
Sprache:	ger
Schlagworte:	APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY CHEMISTRY COMBINATORIAL CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES DIAGNOSIS ENZYMOLOGY HUMAN NECESSITIES HYGIENE IDENTIFICATION LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS SURGERY THEIR RELEVANT APPARATUS TRANSPORTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	It is known to carry out PCR amplification and detection using a reaction vessel in which solid particles are fixed and liquid sample and reagents are moved past these particles. Described herein is a device (10, 30) and method for carrying out such reactions. Solids (for example, beads (16)) are used to obtain separation between bound and "free" label reagents, and these are transferred from a first chamber (12) to a second chamber (32), specifically through a wash liquid so as to wash off the "free" label reagent from the solids (16). The first chamber (12) has a cover (14) which is pierced by prong (40) mounted on cover portion (34) of the second chamber (32) to create passageways (36) for the transfer of the solids (16) from the first chamber (12) to the second chamber (32). The passageways (36) remain contained within the device (10, 30) confining the reaction and detection products therein.