Verfahren für die Detektion von spezifische mRNS und DNS in Zellen

This invention relates to a process for detecting the presence and measuring the quantity of specific mRNA sequences present in in vivo cells or cells maintained in vitro. The process of this invention is applicable to the screening of procaryotic and eucaryotic organisms including the screening of...

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Bibliographische Detailangaben
Hauptverfasser:	PEREIRA, DENNIS A., STONINGTON, CONNECTICUT 06378, US, DAVIDSON, RALPH E., NORTH STONINGTON, CONNECTICUT 06359, US, BANKER, MICHAEL J., GROTON, CONNECTICUT 06340, US
Format:	Patent
Sprache:	ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	This invention relates to a process for detecting the presence and measuring the quantity of specific mRNA sequences present in in vivo cells or cells maintained in vitro. The process of this invention is applicable to the screening of procaryotic and eucaryotic organisms including the screening of human beings for the presence of disease states. The process of this invention is also applicable to the in vitro screening of the effect or effects of chemical compounds upon one or several gene products as exhibited by the presence and amount of mRNA resulting from transcription of said gene or genes. The process of this invention is particularly suited for screening of a large number of compounds for the effect or effects of compounds upon gene products. This invention also relates to compounds capable of affecting the presence of specific mRNA sequences in cells. The process of this invention also is applicable to the identification of novel gene constructs in viruses, microorganisms, plants and animals. This invention also relates to a novel process for the isolation of RNA and DNA from cells, by lysing them in water heated to about 99 DEG C.