Vorrichtung zur Überwachung mikrobieller Aktivität

This invention presents methods for detection and evaluation of metabolic activity of microorganisms based upon their ability to consume dissolved oxygen. The methods utilize a fluorescence detection system which makes use of the sensitivity of the fluorescent emission of certain compounds to the pr...

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Hauptverfasser: MONTHONY, JAMES F., BALTIMORE, MARYLAND 21209, US, HU, KWOK-YU, ELLICOTT CITY, MARYLAND 21042, US, STITT, DAVID T., FREELAND, MARYLAND 21053, US, BURRELL, GREGORY J., RED LION, PENNSYLVANIA 17356, US, SAPITOWICZ, ROBERT, ABINGDON, MARYLAND 21009, US
Format: Patent
Sprache:ger
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Zusammenfassung:This invention presents methods for detection and evaluation of metabolic activity of microorganisms based upon their ability to consume dissolved oxygen. The methods utilize a fluorescence detection system which makes use of the sensitivity of the fluorescent emission of certain compounds to the presence of oxygen, which quenches (diminishes) the compound's fluorescent emission in a concentration dependent manner. Respiring microorganisms will affect the oxygen concentration of a liquid medium in which they are immersed. Thus, this invention provides a convenient system to gather information on the presence, identification and metabolic activity of microorganisms by determining their effect on the oxygen concentration of the media in which they are present.