VERFAHREN ZUR POLYMERASEAKTIVITÄTSBESTIMMUNG

The invention relates to a method of quantitatively determining nucleic acid polymerase activity in a sample, comprising incubation of the sample with a natural or completely or partially synthetic polynucleotide template and a reagent solution containing the necessary substrates including at least...

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Bibliographische Detailangaben
Hauptverfasser:	NEUMUELLER, THORD, MAGNUS, S-752 57 UPPSALA, SE, KAELLANDER, CLAS, FREDRIK, RUNESSON, S-753 37 UPPSALA, SE, WELTMAN, EVA, HELENA, S-753 28 UPPSALA, SE, KARLSTROEM, ROLF, ANDERS, S-753 27 UPPSALA, SE, HAGSTROEM, ASA, MARGARETA, S-754 34 UPPSALA, SE, LANGSTROEM-PERSSON, ULLA, M., S-740 22 BAELINGE, SE, GRONOWITZ, JAN-SIMON, S-752 62 UPPSALA, SE
Format:	Patent
Sprache:	ger
Schlagworte:	ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS,e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES,DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS CONVEYING DERIVATIVES THEREOF ENZYMOLOGY HANDLING THIN OR FILAMENTARY MATERIAL INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MAKING ARTICLES OF PAPER, CARDBOARD OR MATERIAL WORKED IN A MANNERANALOGOUS TO PAPER MAKING CONTAINERS OF PAPER, CARDBOARD OR MATERIAL WORKED IN AMANNER ANALOGOUS TO PAPER MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PACKAGES PACKAGING ELEMENTS PACKING PERFORMING OPERATIONS PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDEDFOR SHAPING OR JOINING OF PLASTICS SPIRITS STORING SUGARS TESTING TRANSPORTING VINEGAR WINE WORKING OF PLASTICS WORKING OF SUBSTANCES IN A PLASTIC STATE, IN GENERAL WORKING PAPER, CARDBOARD OR MATERIAL WORKED IN A MANNER ANALOGOUSTO PAPER
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Beschreibung
Zusammenfassung:	The invention relates to a method of quantitatively determining nucleic acid polymerase activity in a sample, comprising incubation of the sample with a natural or completely or partially synthetic polynucleotide template and a reagent solution containing the necessary substrates including at least one radiolabeled nucleoside triphosphate complementary to the template, separation of the template from the substrate, and measurement of the radioactivity incorporated into the template, which radioactivity is substantially proportional to the polymerase activity in the sample, and wherein, when a primer is required, the latter is either hybridized to the template from the start or added during the determination. According to the invention the template is immobilized to a carrier, said nucleoside triphosphate is labeled with a gamma-radiating isotope, and the measurement of the incorporated radioactivity is performed directly on the immobilized template without release thereof from the carrier. The invention also relates to a kit for determining of nucleic acid polymerase activity, comprising a carrier-bound template and a substrate labeled with a gamma-radiating isotope.