IMMUNO-REAL-TIME-PCR UNTER VERWENDUNG EINER CHIMÄREN DNA ALS AMPLIFIKATIONSMARKER

IMMUNO-REAL-TIME-PCR UNTER VERWENDUNG EINER CHIMÄREN DNA ALS AMPLIFIKATIONSMARKER

The invention concerns a method using PCR, in particular immuno-PCR, for detecting microbiological entities and in particular elements or target molecules. The detection method comprises a step which consists in fixing an entity A, more particularly an antigen, directly or via a capture agent, for e...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: HEINEN, ERNST, MELEN, LAURENCE, EL MOUALIJ, BENAISSA, ZORZI, WILLY, ZORZI, DANIELE
Format: Patent
Sprache:ger
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PHYSICS
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
TESTING
VINEGAR
WINE
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Beschreibung
Zusammenfassung:The invention concerns a method using PCR, in particular immuno-PCR, for detecting microbiological entities and in particular elements or target molecules. The detection method comprises a step which consists in fixing an entity A, more particularly an antigen, directly or via a capture agent, for example an antibody, to one or several surface(s) inside the receptacle(s) of a real time PCR measuring apparatus and a step which consists in associating with said element A a coupling system with a nucleic acid, for example a DNA marker, a step which consists in amplifying the marker nucleic acid. The successive measurements are carried out during the phase amplifying the marker nucleic acid, in the presence of a capture agent and other optional intermediate fixing agents. The detection method is quantitative and can be used in particular for prion polypeptide assay.