Turbidimetric determn. of fibrin monomers - in plasma, by pptn. with netropsin

Turbidimetric determn. of fibrin monomers in plasma is effected by (a) diluting citrated plasma with at least an equal vol. of Owrens veronal buffer, (b) treating 4 parts of the diluted plasma with 1 part of a 15 mg/ml netropsin soln. in 0.15M Hepes buffer (pH 7.5), (c) measuring the extinction at 4...

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Hauptverfasser:	FUNKE, UDO., O-8800 ZITTAU, DE, SEIFERT, ANDREAS., O-8900 GOERLITZ, DE, TOEPFER, GOTTFRIED., O-8909 GOERLITZ, DE, SAUER, CORNELIUS., O-8909 GOERLITZ, DE
Format:	Patent
Sprache:	eng ; ger
Schlagworte:	INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING PHYSICS TESTING
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Beschreibung
Zusammenfassung:	Turbidimetric determn. of fibrin monomers in plasma is effected by (a) diluting citrated plasma with at least an equal vol. of Owrens veronal buffer, (b) treating 4 parts of the diluted plasma with 1 part of a 15 mg/ml netropsin soln. in 0.15M Hepes buffer (pH 7.5), (c) measuring the extinction at 405 nm after 5 and 600 secs., and (d) reading the result from a calibration curve for fibrin monomer concn. versus extinction difference. USE/ADVANTAGE - The process may be used in the diagnosis of blood clotting disorders associated with disseminated intravasal coagulation. The process is rapid and gives quantitative results (cf. EP-253255)