ELISA determn. of low mol. wt. pesticides, antibiotics or toxins - using enzyme labelled antibody reactant, providing lower detection limit

In the quantitative ELISA determn. of low mol. wt. organic substances (I), the new feature is that an enzyme-coupled antibody (Ab1) is used as the reaction partner. Ab1 is coupled to alkaline phosphatase using glutardialdehyde as coupling agent. It is directed against ochratoxin A, aflatoxins M1 or B1; chloramphenicol; zearalenone; triazines; deoxynivalenol; dinitro aromatic cpds.; ergot alkaloids; nitrosamines, penicillins; patulin; phenylureas, or chlorinated anilines or phenols. USE/ADVANTAGE - This method provides significantly better detection limits (up to 100 times lower) than the conventional process which uses labelled hapten. It is also quicker and less expensive, since derivatisation steps are avoided. The method is esp. used to monitor mycotoxins, antibodies and pesticides in food, water, etc.. Transportable, storage-stable assay kits can be prepd..