Selection of yeast strains producing di:carboxylic acids - by culturing on medium contg. alkane and alkyne

Selection of yeast strains capable of producing dicarboxylic acids from alkanes is effected by culturing a yeast cell suspension on a nutrient medium contg. an 8-20C alkane as C source and a 3-20C alkylene as inhibitor, selecting the best-growing colonies, and opt. repeating this procedure one or mo...

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Hauptverfasser: EIERDANZ, HORST., 4010 HILDEN, DE, GIESEL-BUEHLER, HERMINE., 7913 SENDEN, DE, MEUSSDOERFFER, FRANZ, HAENGGI, URS., LINZ, AT, SCHINDLER, JOACHIM., 4010 HILDEN, DE
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Sprache:eng ; ger
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Zusammenfassung:Selection of yeast strains capable of producing dicarboxylic acids from alkanes is effected by culturing a yeast cell suspension on a nutrient medium contg. an 8-20C alkane as C source and a 3-20C alkylene as inhibitor, selecting the best-growing colonies, and opt. repeating this procedure one or more times. The inhibitor is 1-dodecyne (I). The yeast is Candida tropicalis or C.lipolytica and is subjected to mutagenesis before selection. ADVANTAGE - The method is more specific and gives high success rates tan prior art selection methods (cf. DE373812).