High yield L-Arginine prodn. - by cultivation of Brevibacterium or Corynebacterium species resistant to aspartic acid analogue(s)

High yield L-Arginine prodn. - by cultivation of Brevibacterium or Corynebacterium species resistant to aspartic acid analogue(s)

Aspartic acid analogue suppresses the growth of the microorganism, but the suppression is lowered in the presence of aspartic acid. The microorganism is derived from such a stock as Brevibacterium divaricatum ATCC 14020, Bre. flavum ATCC 14067, Bre. lactofermentum ATCC 13869, Corynebacterium acetoac...

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Bibliographische Detailangaben
Hauptverfasser: NAKAMURA,YAYOI, AKASHI,KUNIHIKO, IKEDA,SHIGEHO, TSUCHIDA,TAKAYASU, YOSHII,HIROE
Format: Patent
Sprache:eng ; ger
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:Aspartic acid analogue suppresses the growth of the microorganism, but the suppression is lowered in the presence of aspartic acid. The microorganism is derived from such a stock as Brevibacterium divaricatum ATCC 14020, Bre. flavum ATCC 14067, Bre. lactofermentum ATCC 13869, Corynebacterium acetoacidophilum ATCC 13870, etc. by treating the stock with N-methyl-N'-nitro-N-nitroso guanidine-soln. (250 micro g/ml) at 30 deg. C for 30 min., and screening the stock having resistance to aspartic-acid analogues. The microorganism is cultivated in a medium contg. a C source e.g. glucose, sucrose, starch, organic acid, alcohol, etc. a N source, e.g. ammonium salt, ammonia gas, urea, etc. and other nutrients, at pH 5-9 at 24-37 deg.C for 2-7 days.