VERFAHREN UND MITTEL ZUR BESTIMMUNG VON TRIJOD-THYRONIN

A composition for diagnostic use is disclosed, more particularly for the dosage of tri-iodo thyronine in human blood serum: the subject composition comprises tri-iode thyronine as such and an enzyme which is covalently bonded to tri-iodo thyronine. A diagnostic method is also disclosed, which is int...

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Hauptverfasser: BARONCELLI,VITTORIO, RENZI,PIERLUIGI
Format: Patent
Sprache:ger
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Beschreibung
Zusammenfassung:A composition for diagnostic use is disclosed, more particularly for the dosage of tri-iodo thyronine in human blood serum: the subject composition comprises tri-iode thyronine as such and an enzyme which is covalently bonded to tri-iodo thyronine. A diagnostic method is also disclosed, which is intended for using the above specified composition in diagnostic runs. The method, briefly resumed, consists removing the tri-iodo thyronine of the serum sample being assayed by means of an insolubilized anti-T3 antibody and treating the residue with the sensibilized enzyme which is nothing else than the covalent formed by tri-iodo thyronine and an appropriate enzyme, such as carbonic anhydrase.