SUBSTRAT ZUR PROTEINASEBESTIMMUNG
1496907 Determination of proteinase BEHRINGWERKE AG 23 Dec 1974 [21 Dec 1973] 55521/74 Heading G1B [Also in Division C3] A method of determining proteinase comprises incubating the proteinase with a substrate com- prising a protein covalently bound to a solid carrier and having a reactive dyestuff c...
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Zusammenfassung: | 1496907 Determination of proteinase BEHRINGWERKE AG 23 Dec 1974 [21 Dec 1973] 55521/74 Heading G1B [Also in Division C3] A method of determining proteinase comprises incubating the proteinase with a substrate com- prising a protein covalently bound to a solid carrier and having a reactive dyestuff covalently bonded to the protein [see Division C3], removing any undergraded substrate by filtration or centrifugation, measuring the absorption of the filtrate or centrifugate at a wavelength or wavelengths determined by the absorption characteristics of the reactive dyestuff, and calculating the activity of the enzyme therefrom. |
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