Acarviosyl-Transferase aus Actinoplanes sp. SE 50/110 und -Mutanten, Verfahren zur Herstellung sowie zur Verwendung von Acarviosyl-Transferase bei der Umwandlung von Acarbose-Homologen in Acarbose, zur Herstellung von Acarbose-Homologen und zur Stamm-Optimierung

The invention relates to acarviosyl transferase from actinomycetes, mainly from Actinoplanes sp. SE 50/110 and its mutants, to a process for isolating, purifying and characterizing the enzyme, to the isolation and characterization of the acbD gene encoding the acarviosyl transferase, to the expressi...

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Hauptverfasser:	SCHROEDER, WERNER., 42113 WUPPERTAL, DE, LENZ, JUERGEN., 51373 LEVERKUSEN, DE, PAPE, HERMANN, DR., 48158 MUENSTER, DE, PIEPERSBERG, WOLFGANG, DR., 42349 WUPPERTAL, DE, CRUEGER, ANNELIESE., 40699 ERKRATH, DE, DELLWEG, HANS-GEORG., 42113 WUPPERTAL, DE, STRATMANN, ANSGAR, 45527 HATTINGEN, DE, DISTLER, JUERGEN., 42107 WUPPERTAL, DE, GOEKE, KLAUS., 58708 MENDEN, DE, HEMKER, MICHAEL, 48151 MUENSTER, DE, SCHAPER, BEATE., 48151 MUENSTER, DE
Format:	Patent
Sprache:	ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to acarviosyl transferase from actinomycetes, mainly from Actinoplanes sp. SE 50/110 and its mutants, to a process for isolating, purifying and characterizing the enzyme, to the isolation and characterization of the acbD gene encoding the acarviosyl transferase, to the expression of the acarviosyl transferase in a heterologous host organism, to the use of the acarviosyl transferase for converting acarbose minor constituents into acarbose or for preparing acarbose homologues, to the use of the acarviosyl transferase in acarbose purification, and also to the preparation of production mutants in which formation of minor constituents is reduced by means of inactivation of the acarviosyl transferase gene.