Enzymatisch-elektrochemischer Einschritt-Affinitätssensor zur quantitativen Bestimmung von Analyten in wäßrigen Medien und Affinitätsassay

The invention relates to an enzymatic-electrochemical affinity sensor and a one-shot affinity assay for the quantitative determination of analytes in aqueous media. The invention relates particularly to an enzymatic-electrochemical signal amplification system for the highly sensitive indication of a...

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Bibliographische Detailangaben
Hauptverfasser:	KOPINKE, HOLM., 04357 LEIPZIG, DE, GRUENDIG, BERND.NAT., 04318 LEIPZIG, DE, RENNEBERG, ILKA., 13187 BERLIN, DE, STREHLITZ, BEATE., 04129 LEIPZIG, DE
Format:	Patent
Sprache:	ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to an enzymatic-electrochemical affinity sensor and a one-shot affinity assay for the quantitative determination of analytes in aqueous media. The invention relates particularly to an enzymatic-electrochemical signal amplification system for the highly sensitive indication of affinity reactions and is especially suitable in the form of a one-shot affinity sensor for on-site analysis. The object of the invention is also the use of phenol oxidase as a marker enzyme for the affine bond partners in an electrochemical affinity sensor or assay and the use of an enzyme hydrolysing phenolic compounds as a marker enzyme for the affine bond partners in combination with a phenol oxidase as a catalyst for the amplification reaction in an electrochemical affinity assay.