Thermostabile DNA-Polymerase aus Thermococcus spec

This invention concerns a thermostable enzyme with DNA polymerase and 3'-5'-exonuclease activity obtainable from Thermococcus spec. TY (DSM 10597); the underlying genomic DNA corresponds to the nucleic acid sequences reported in SEQ. ID NO:2 and SEQ. ID NO:4. The invention concerns, in add...

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Bibliographische Detailangaben
Hauptverfasser: ANTRANIKIAN, GARABED., 21218 SEEVETAL, DE, NIEHAUS, FRANK-BIOL., 23558 LUEBECK, DE, FREY, BRUNO-BIOL. DR., 82377 PENZBERG, DE
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Sprache:ger
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Zusammenfassung:This invention concerns a thermostable enzyme with DNA polymerase and 3'-5'-exonuclease activity obtainable from Thermococcus spec. TY (DSM 10597); the underlying genomic DNA corresponds to the nucleic acid sequences reported in SEQ. ID NO:2 and SEQ. ID NO:4. The invention concerns, in addition, a process for producing the recombinant thermophile enzyme, as well as its use for specific duplication of nucleic acid sequences.