Isothermal cyclic amplification of nucleic acid, useful e.g. for detecting mutations by the arrayed primer extension method, comprises that helicase is used for separation of nucleic acid strands

Method for cyclic amplification of nucleic acid that uses helicase activity to unwind nucleic acid double strands, then hydridization of the individual strands to oligonucleotides (ON), extension of ON by using polymerase activity, to produce double strands, and repetition of the procedure. An indep...

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Bibliographische Detailangaben
1. Verfasser:	PLACHOV, DIMITRIJ
Format:	Patent
Sprache:	eng ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Method for cyclic amplification of nucleic acid that uses helicase activity to unwind nucleic acid double strands, then hydridization of the individual strands to oligonucleotides (ON), extension of ON by using polymerase activity, to produce double strands, and repetition of the procedure. An independent claim is also included for a kit for the process that contains a peptide with helicase activity. Die Erfindung betrifft ein Verfahren zur zyklischen Amplifikation von Nukleinsäuren, bei dem jeder neue Zyklus durch ein Peptid oder Protein mit einer Helicase-Aktivität initiiert wird.