Preparation of N-carbamoylamino acid, useful as intermediate in enantioselective synthesis of amino acids, by enzymatic hydrolysis of the corresponding amide

Preparing N-carbamoylamino acid (I) by reacting an N-carbamoylamino acid amide (II) in presence of a hydrolase (III), is new. Independent claims are also included for the following: (1) preparing amino acids (IV) from (I) or hydantoin that includes the new preparation of (I); (2) preparing (IV) from...

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Hauptverfasser: MAY, OLIVER, DINGERDISSEN, UWE, TRAUTHWEIN, HARALD, SACHERER, CHRISTIANE
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creator MAY, OLIVER
DINGERDISSEN, UWE
TRAUTHWEIN, HARALD
SACHERER, CHRISTIANE
description Preparing N-carbamoylamino acid (I) by reacting an N-carbamoylamino acid amide (II) in presence of a hydrolase (III), is new. Independent claims are also included for the following: (1) preparing amino acids (IV) from (I) or hydantoin that includes the new preparation of (I); (2) preparing (IV) from (II) comprising reaction of (II) to (I) and/or enantioselective reaction of (I) to free (IV) that includes the new preparation of (I); and (3) whole cell catalyst (A) comprising cloned genes for (III), a carbamoylase, (V), a hydantoinase (VI) and optionally a hydantoin racemase (VII). Die vorliegende Erfindung betrifft ein Verfahren zur Herstellung von N-Carbamoylaminosäuren durch enzymatische Umsetzung von N-Carbamoylaminosäureamiden in Gegenwart einer Hydrolase.
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title Preparation of N-carbamoylamino acid, useful as intermediate in enantioselective synthesis of amino acids, by enzymatic hydrolysis of the corresponding amide
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