Cellular method for identifying modulators of target molecules, useful in screening for pharmaceuticals, from effects of test compounds on both cell growth and reporter expression

A cellular method for identifying compounds (I) that can modify the activity of a target molecule (II) which affects cell replication, is new. At least one cell, containing a reporter gene (RG) is treated with test compounds, then both cell replication and activity of the RG product (III) are determ...

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Bibliographische Detailangaben
Hauptverfasser:	MAI, BERNHARD, DE HOOP, MELTSJE, GRATZER, SABINE
Format:	Patent
Sprache:	eng ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	A cellular method for identifying compounds (I) that can modify the activity of a target molecule (II) which affects cell replication, is new. At least one cell, containing a reporter gene (RG) is treated with test compounds, then both cell replication and activity of the RG product (III) are determined. Die Erfindung betrifft ein zelluläres Verfahren zur Identifizierung von Substanzen, die fähig sind, die Aktivität eines Zielmoleküls zu beeinflussen, wobei die zu analysierenden Zellen mindestens ein Reportergen tragen, und die Aktivität des Zielmoleküls sich auf die Zellvermehrung auswirkt.