Marker substance for use in detection reagent of immunoassay for specific detection of biomaterial, has hetero element, subunit that is peptide, and reporter group that comprises chelating agent, complexing agent and ion exchanger

The marker substance has a reactive group and a carrier material having two pieces of a subunit. The pieces are connected with one another by a peptide bond. The sub-unit has amino acid with a hetero element-containing reporter groups. The hetero element is any chemical element other than hydrogen, carbon, nitrogen and oxygen. The subunit is a peptide. The reporter group comprises a chelating agent, a complexing agent and ion exchanger. A substrate is provided a thiocarbonyl group or a thioether bond. Independent claims are included for the following: (1) a method for preparing a marker substance; (2) a detection reagent comprises molecule or fragment of a molecule selected under an antibody that comprises immunoglobulin G, immunoglobulin M, immunoglobulin E, avidin, biotin, enzyme, enzyme substrate, hormone, protein A, protein G, receptor and streptavidin; (3) an analysis method for qualitative and quantitative detection of an analyte in a sample; (4) an immunoassay selected from enzyme immunoassay, enzyme-linked immunosorbent assay, radioimmunoassay, immunoblot, western blot, southern blot or microarray; and (5) a method for retracking and covered individual marking of a biomolecule. Markersubstanz mit einer reaktiven Gruppe, wobei die Markersubstanz für den spezifischen Nachweis von Biomaterial angepasst ist und ein Trägermaterial (1) umfasst, das zumindest zwei Stück einer Untereinheit enthält, die miteinander über eine Peptidbindung (100) verknüpft sind, wobei die Untereinheit zumindest eine Aminosäure mit einer Heteroelement-haltigen Reportergruppe (10, 20) aufweist, und das Heteroelement ein beliebiges chemisches Element außer Wasserstoff (H), Kohlenstoff (C), Stickstoff (N) und Sauerstoff (O) ist.