Detecting the bacterium Taylorella equigenitalis comprises nucleic acid amplification using two defined oligonucleotide primers

Detecting the bacterium Taylorella equigenitalis comprises nucleic acid amplification using an oligonucleotide comprising at least 15 consecutive bases of a first sequence (seq1) and oligonucleotide comprising at least 15 consecutive bases of a second sequence (seq2). Detecting the bacterium Taylorella equigenitalis comprises nucleic acid amplification using an oligonucleotide comprising at least 15 consecutive bases of a first sequence (seq1) and oligonucleotide comprising at least 15 consecutive bases of a second sequence (seq2). 5'-TTGTCAGGGAAGAAAAGGTTTGTGT-3' (seq1); and 5'-GGATTTACATCTCTCTTTCCGAA-3' (seq2). Independent claims are also included for: (1) oligonucleotide (I) comprising at least 15 consecutive bases of seq1; (2) oligonucleotide (II) comprising at least 15 consecutive bases of seq2; (3) nucleic acid (III) comprising a third sequence (seq3) and/or a fourth sequence (seq4); (4) nucleic acid (IV) comprising a fifth sequence (not given in the specification); (5) composition comprising (I) and/or (II); (6) kit for amplifying a nucleic acid of Taylorella equigenitalis, comprising (I) and (II). 5'-TTGTCAGGGAAGAAAAGGTTTGTGTGGCCGAGTTCTATCTGAATCCTGA-3' (seq3); and 5'-GGATTTACATCTCTCTTTCCGAAGAGCGCTTTGTCATGATTTCCAGG-3' (seq4). Die Erfindung betrifft ein Verfahren zum spezifischen Nachweis des Bakteriums Taylorella equigenitalis durch Amplifikation eines Abschnittes der bakteriellen DNA, wobei die Koamplifikation eines internen, artifiziellen Standards besondere Vorteile bietet. In der klinisch-diagnostischen Anwendung ist das Verfahren vor allem deswegen überlegen, weil es gegenüber sehr nahe verwandten Bakterien, wie Taylorella asinigenitalis, zu differenzieren vermag. Die vorliegende Erfindung betrifft des Weiteren bestimmte Oligonukleotide, Nukleinsäuren, deren Verwendung und diese enthaltende Zusammensetzungen sowie entsprechende Analysekits.