Verfahren zur quantitativen Bestimmung von Dicarbonylverbindungen

The invention relates to a method for quantitative determination of alpha oxoaldehydes, especially glyoxal, methylglyoxal and 3-deoxyglucuron in liquid and gaseous samples. The method comprises two steps: the oxoaldehydes are enriched and mixed with a detection agent containing a fluorophore. The en...

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Bibliographische Detailangaben
Hauptverfasser:	KEY, GOERAN, SCHREIBER, SABINE, SCHULZE-PELLENGAHR, UTA, PEYRER, HARALD, DETERS, HELGA, KLEIBOEHMER, WOLFGANG
Format:	Patent
Sprache:	ger
Schlagworte:	GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING PHYSICS TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION TECHNICAL SUBJECTS COVERED BY FORMER USPC TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS TESTING
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Beschreibung
Zusammenfassung:	The invention relates to a method for quantitative determination of alpha oxoaldehydes, especially glyoxal, methylglyoxal and 3-deoxyglucuron in liquid and gaseous samples. The method comprises two steps: the oxoaldehydes are enriched and mixed with a detection agent containing a fluorophore. The enrichment step consists in reacting oxoaldehydes with reactive groups (aminopyrazines, aminopyridines, aminopiperidines or aminoguanidines) which are immobilized on a solid phase or a water-soluble polymer. The bound aldehydes are subsequently reacted in a second step with a specific detection agent (a flurophoric antibody). Die vorliegende Erfindung betrifft ein Verfahren zur quantitativen Bestimmung von Dicarbonylverbindungen in gasförmigen und/oder flüssigen Proben sowie Aerosolen. Hierdurch kann das Verfahren auch zur Bestimmung der Akkumulation von reaktiven Carbonylverbindungen beim sogenannten Carbonyl-Streß von Diabetikern angewendet werden.