Specifically detecting microorganisms in a sample, by polymerase chain reaction with reaction and competitor primers, useful for detecting subspecies of Listeria, in particular Listeria monocytogenes

Specifically detecting microorganisms in a sample by Polymerase Chain Reaction (PCR) where in addition to reaction primers specific to the target organism, competition primers specific for non-target organisms are also used, is new. An Independent claims is also included for a DNA primer developed o...

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Hauptverfasser:	WAGNER, MICHAEL, WALCHER, MARION, SNAIDR, JIRI
Format:	Patent
Sprache:	eng ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Specifically detecting microorganisms in a sample by Polymerase Chain Reaction (PCR) where in addition to reaction primers specific to the target organism, competition primers specific for non-target organisms are also used, is new. An Independent claims is also included for a DNA primer developed on the basis of structural or functional genes of the microorganism where the genes exhibit both specific ranges and master specific ranges. Die Erfindung betrifft ein Verfahren zum spezifischen Nachweis von nahe verwandten Mikroorganismen in einer Probe durch Polymerase-Kettenreaktion (PCR). Insbesondere betrifft die Erfindung ein Verfahren zum spezifischen Nachweis von Mikroorganismen der Gattung Listeria unterhalb der Artebene sowie Primer, die bei diesem Verfahren eingesetzt werden können.