VERFAHREN ZUR HERSTELLUNG EINER ZELLFREIEN ASPARTASE-LOESUNG

Aspartic acid is enzymatically produced from ammonium fumarate in the presence of the enzyme, aspartase. A cell-free aspartase solution is prepared by forming an aqueous cell suspension of aspartase- containing microbial cells; incubating the cell suspension, centrifuging the cell suspension to form...

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Bibliographische Detailangaben
Hauptverfasser:	MOSER,LOIS J.,US, STERNBERG,DAVID C.,US
Format:	Patent
Sprache:	ger
Schlagworte:	APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Aspartic acid is enzymatically produced from ammonium fumarate in the presence of the enzyme, aspartase. A cell-free aspartase solution is prepared by forming an aqueous cell suspension of aspartase- containing microbial cells; incubating the cell suspension, centrifuging the cell suspension to form a clarified solution that contains a major portion of the aspartase activity originally present in the cells, a sufficient amount of an aspartase-stabilizing salt being included in the cell suspension or to the clarified solution, to make the clarified solution hypertonic. Aspartase recovered in this manner is particularly useful in immobilized enzyme reactors for the production of aspartic acid.