Hyaluronate-lyase enzyme, process of its preparation, use and process for preparing low-molecular hyaluronate

Resení se týká enzymu schopného stepit kyselinu hyaluronovou, který je produkován houbami rodu Fistulina (zejména Fistulina hepatica). Degradace probíhá lyázovým mechanismem, pri nemz vznikají dvojné vazby mezi C4 a C5 kyseliny glukuronové. Dále je popsán zpusob prípravy a cistení enzymu a jeho mozné praktické vyuzití pro prípravu nízkomolekulárního hyaluronanu nebo kosmetických ci farmaceutických prostredku. Resení se rovnez týká zpusobu prípravy nízkomolekulárního hyaluronanu. The present invention relates to an enzyme that is capable of cleaving hyaluronic acid and that is produced by mushrooms of the Fistulina species (especially Fistulina hepatica mushroom). The degradation takes place through lyase mechanism characterized by the formation of double bonds between C4 and C5 of glucuronic acid. There is also disclosed a process for preparing and purification of the above-described enzyme as well as possible practical use thereof for the preparation of a low-molecular hyaluronate or cosmetic or pharmaceutical compositions. The present invention also relates to a process for preparing a low-molecular hyaluronate.