Extraction process of periplasmatic protein
In the present invention there is disclosed a process of extracting periplasmatic protein and comprising (i) cultivation of prokaryotic micro-organism being transformed by means of expression vector containing a gene encoding said protein and means for expression thereof in periplasm of said micro-o...
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| creator | LEGOUX RICHARD SALOME MARC MALDONADO PAUL |
| description | In the present invention there is disclosed a process of extracting periplasmatic protein and comprising (i) cultivation of prokaryotic micro-organism being transformed by means of expression vector containing a gene encoding said protein and means for expression thereof in periplasm of said micro-organism, (ii) centrifugation or micro-filtration of the obtained cultivation mixture in order to obtain a cellular fraction, optionally lysis of cells of the obtained cellular fraction and centrifugation of the obtained lysate in order to obtain a disintegrated cellular fraction, (iii) suspending the obtained cellular fraction or the disintegrated cellular fraction in a buffer solution, (iv) centrifugation of the obtained suspension in order to obtain a supernatant and (v) isolation of said protein from this supernatant. The invented process is characterized in that the buffer solution contains arginine. |
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The invented process is characterized in that the buffer solution contains arginine.</description><edition>7</edition><language>eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; DERIVATIVES THEREOF ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; NUCLEIC ACIDS ; NUCLEOSIDES ; NUCLEOTIDES ; ORGANIC CHEMISTRY ; PEPTIDES ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; SUGARS ; VINEGAR ; WINE</subject><creationdate>2000</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20000412&DB=EPODOC&CC=CZ&NR=286509B6$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20000412&DB=EPODOC&CC=CZ&NR=286509B6$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>LEGOUX RICHARD</creatorcontrib><creatorcontrib>SALOME MARC</creatorcontrib><creatorcontrib>MALDONADO PAUL</creatorcontrib><title>Extraction process of periplasmatic protein</title><description>In the present invention there is disclosed a process of extracting periplasmatic protein and comprising (i) cultivation of prokaryotic micro-organism being transformed by means of expression vector containing a gene encoding said protein and means for expression thereof in periplasm of said micro-organism, (ii) centrifugation or micro-filtration of the obtained cultivation mixture in order to obtain a cellular fraction, optionally lysis of cells of the obtained cellular fraction and centrifugation of the obtained lysate in order to obtain a disintegrated cellular fraction, (iii) suspending the obtained cellular fraction or the disintegrated cellular fraction in a buffer solution, (iv) centrifugation of the obtained suspension in order to obtain a supernatant and (v) isolation of said protein from this supernatant. The invented process is characterized in that the buffer solution contains arginine.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>DERIVATIVES THEREOF</subject><subject>ENZYMOLOGY</subject><subject>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>NUCLEIC ACIDS</subject><subject>NUCLEOSIDES</subject><subject>NUCLEOTIDES</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>SUGARS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2000</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZNB2rSgpSkwuyczPUygoyk9OLS5WyE9TKEgtyizISSzOTSzJTAZJlKRm5vEwsKYl5hSn8kJpbgZ5N9cQZw_d1IL8-NTigsTk1LzUknjnKCMLM1MDSyczY8IqAALTKVQ</recordid><startdate>20000412</startdate><enddate>20000412</enddate><creator>LEGOUX RICHARD</creator><creator>SALOME MARC</creator><creator>MALDONADO PAUL</creator><scope>EVB</scope></search><sort><creationdate>20000412</creationdate><title>Extraction process of periplasmatic protein</title><author>LEGOUX RICHARD ; SALOME MARC ; MALDONADO PAUL</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CZ286509B63</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng</language><creationdate>2000</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>DERIVATIVES THEREOF</topic><topic>ENZYMOLOGY</topic><topic>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>NUCLEIC ACIDS</topic><topic>NUCLEOSIDES</topic><topic>NUCLEOTIDES</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>SUGARS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>LEGOUX RICHARD</creatorcontrib><creatorcontrib>SALOME MARC</creatorcontrib><creatorcontrib>MALDONADO PAUL</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>LEGOUX RICHARD</au><au>SALOME MARC</au><au>MALDONADO PAUL</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Extraction process of periplasmatic protein</title><date>2000-04-12</date><risdate>2000</risdate><abstract>In the present invention there is disclosed a process of extracting periplasmatic protein and comprising (i) cultivation of prokaryotic micro-organism being transformed by means of expression vector containing a gene encoding said protein and means for expression thereof in periplasm of said micro-organism, (ii) centrifugation or micro-filtration of the obtained cultivation mixture in order to obtain a cellular fraction, optionally lysis of cells of the obtained cellular fraction and centrifugation of the obtained lysate in order to obtain a disintegrated cellular fraction, (iii) suspending the obtained cellular fraction or the disintegrated cellular fraction in a buffer solution, (iv) centrifugation of the obtained suspension in order to obtain a supernatant and (v) isolation of said protein from this supernatant. The invented process is characterized in that the buffer solution contains arginine.</abstract><edition>7</edition><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PEPTIDES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS VINEGAR WINE |
| title | Extraction process of periplasmatic protein |
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