Extraction process of periplasmatic protein

In the present invention there is disclosed a process of extracting periplasmatic protein and comprising (i) cultivation of prokaryotic micro-organism being transformed by means of expression vector containing a gene encoding said protein and means for expression thereof in periplasm of said micro-o...

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Bibliographische Detailangaben
Hauptverfasser:	LEGOUX RICHARD, SALOME MARC, MALDONADO PAUL
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PEPTIDES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS VINEGAR WINE
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Beschreibung
Zusammenfassung:	In the present invention there is disclosed a process of extracting periplasmatic protein and comprising (i) cultivation of prokaryotic micro-organism being transformed by means of expression vector containing a gene encoding said protein and means for expression thereof in periplasm of said micro-organism, (ii) centrifugation or micro-filtration of the obtained cultivation mixture in order to obtain a cellular fraction, optionally lysis of cells of the obtained cellular fraction and centrifugation of the obtained lysate in order to obtain a disintegrated cellular fraction, (iii) suspending the obtained cellular fraction or the disintegrated cellular fraction in a buffer solution, (iv) centrifugation of the obtained suspension in order to obtain a supernatant and (v) isolation of said protein from this supernatant. The invented process is characterized in that the buffer solution contains arginine.