PREPARATION OF INMOVILIZED COLINESTERASE ENZYMES AND ITS OBTAINMENT PROCEDURE
PCT No. PCT/HU81/00047 Sec. 371 Date Mar. 8, 1983 Sec. 102(e) Date Mar. 8, 1983 PCT Filed Dec. 23, 1981 PCT Pub. No. WO83/00345 PCT Pub. Date Feb. 3, 1983.Immobilized cholinesterase enzyme preparations are prepared by treating a polymeric resin, built up from acrylic acid and/or methacrylic acid and...
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Zusammenfassung: | PCT No. PCT/HU81/00047 Sec. 371 Date Mar. 8, 1983 Sec. 102(e) Date Mar. 8, 1983 PCT Filed Dec. 23, 1981 PCT Pub. No. WO83/00345 PCT Pub. Date Feb. 3, 1983.Immobilized cholinesterase enzyme preparations are prepared by treating a polymeric resin, built up from acrylic acid and/or methacrylic acid and acryl amide and/or methacryl amide monomers with an acryl or allyl type cross-linking agent and containing at least 0.1 meq/g of -COOH functional groups, with a carbodiimide derivative which is soluble in water or is soluble in an organic solvent at temperatures below 0 DEG C., applying a solution of cholinesterase enzyme with a pH of 4.5 to 8.5 to the resulting activated support, washing the resulting product, and drying it if desired. |
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