METHOD OF HIND II AND HIND III ENZYMES PURIFICATIONS FOR GENIC HANDLINGS

A method of purification of the restrictive endonucleases Hind II and Hind III by means of vertical liquid chromatography, whereby both activities are separated in columns of phosphocellulose or Blue-cellulose or heparin-cellulose, preferably by combination of these columns and possibly with a colum...

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1. Verfasser: PRESS MILAN RNDR.,CS
Format: Patent
Sprache:eng
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Zusammenfassung:A method of purification of the restrictive endonucleases Hind II and Hind III by means of vertical liquid chromatography, whereby both activities are separated in columns of phosphocellulose or Blue-cellulose or heparin-cellulose, preferably by combination of these columns and possibly with a column of DEAE-cellulose, which provides higher effectiveness of separation than with a DEAE-cellulose column alone.