METHOD OF HIGH-PURITY CHYMOPAPAIN PRODUCTION

The solution applies to the production of highly pure chymopapain from raw chymopapain. According to the solution, raw chymopapain dissolves in the presence of a reductant in the water of a buffer. The pure upper part of the dissolution undergoes gel-permeation chromatography and fractions containin...

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Hauptverfasser: GAAL JOZSEF DR.,HU, VIZKELETI TIBOR DR.,HU, VIRAG SANDOR DR.,HU, JOZSA LASZLO DR.,HU, ASBOTH BENCE ,HU, FEJER ERZSEBET ,HU, POLGAR LASZLO ,HU
Format: Patent
Sprache:eng
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Zusammenfassung:The solution applies to the production of highly pure chymopapain from raw chymopapain. According to the solution, raw chymopapain dissolves in the presence of a reductant in the water of a buffer. The pure upper part of the dissolution undergoes gel-permeation chromatography and fractions containing active enzyme are picked and dried by freezing.