Real-time fluorescent quantitative PCR (Polymerase Chain Reaction) chip and PCR system

The utility model discloses a real-time fluorescent quantitation PCR chip and a real-time fluorescent quantitation PCR system, the real-time fluorescent quantitation PCR chip comprises a chip body, the chip body is provided with a sample adding port, a reaction cavity and a liquid flow channel commu...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: YAN JING, ZOU CHANGHUA, CHENG LIN
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The utility model discloses a real-time fluorescent quantitation PCR chip and a real-time fluorescent quantitation PCR system, the real-time fluorescent quantitation PCR chip comprises a chip body, the chip body is provided with a sample adding port, a reaction cavity and a liquid flow channel communicated with the sample adding port and the reaction cavity, the liquid flow channel comprises N circulation channels arranged in sequence, n is a positive integer larger than 1, and each circulation channel is provided with a head end portion located in a high-temperature heating area and a tail end portion located in a low-temperature heating area. The head end part of the ith circulation channel is communicated with the tail end part of the (i-1) th circulation channel, the tail end part of the ith circulation channel is communicated with the head end part of the (i + 1) th circulation channel, and i is a positive integer greater than 1 and less than N; and the tail end part of the Nth circulation channel is com