Low-shear-force perfusion culture system suitable for high-density culture of mammalian cells

The utility model provides a low-shear-force perfusion culture system suitable for high-density culture of mammalian cells, which is characterized in that two ends of a hollow fiber column are connected with a reactor unit, and a lifting unit is arranged below the reactor unit; the two reactors generate a liquid level difference through the lifting unit, a cell-containing culture medium is driven to flow in the hollow fiber column, the flow is controlled through the flow control unit, a fresh culture medium is supplemented through the liquid inlet pump, and a waste culture medium (filtrate) in the outer cavity is pumped out through the liquid outlet pump. The shear force of the perfusion system only comes from the wall friction force of the hollow fiber column and the pipeline, the shear force is low, and damage to cells can be neglected. Through alternate lifting of the two reactors, tangential flow is generated on the surface of a hollow fiber column membrane, a filter membrane is effectively cleaned, and t