Micro-fluidic chip and in-vitro detection device

The utility model discloses a micro-fluidic chip and an in-vitro detection device. The micro-fluidic chip comprises a chip main body, and a compression mechanism, a sample loading mechanism, an anti-reflux one-way mechanism and a plurality of reaction mechanisms which are arranged on the chip main body, the chip main body is provided with a plurality of micro-fluidic channels, the sample loading mechanism comprises a sample tube and a tube cover connected to the sample tube, a sample cavity is communicated with the compression mechanism, the compression mechanism is used for conveying gas into the sample cavity, and the reaction mechanism comprises a reaction chamber and an exhaust hole which is communicated with the reaction chamber and can ventilate with the outside; each reaction chamber is communicated with the sample tube through a micro-fluidic channel, an anti-reflux one-way mechanism is arranged on the micro-fluidic channel, a self-blocking part is arranged in the exhaust hole, and the self-blocking p