A microfluidic chip for polynucleotide sample analysis

The utility model relates to a microfluidic chip or, in particular to the microfluidic chip applied to the analysis of polynucleotide samples. The chip is linked by an upper layer glass substrate and a lower layer glass substrate under a high temperature. The upper layer glass substrate comprises tw...

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Bibliographische Detailangaben
1. Verfasser: BINGCHENG,ZHONG LIN
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:The utility model relates to a microfluidic chip or, in particular to the microfluidic chip applied to the analysis of polynucleotide samples. The chip is linked by an upper layer glass substrate and a lower layer glass substrate under a high temperature. The upper layer glass substrate comprises two or more micro channel reaction tanks and an electrophoresis separating channel which is connected with the reaction tanks, sample outlets are positioned between the reactions tanks and the electrophoresis separating channel, the other ends of the reaction tanks are provided with sample inlets, and sample waste liquor tanks are arranged between the sample outlets and the electrophoresis separating channel. The two ends of the electrophoresis separating channel are respectively provided with a buffer solution tank and a waste liquor tank. the corresponding positions of each reaction tank in the upper layer glass substrate and the lower layer glass substrate are provided with an independent temperature controlling unit, the chip, after being sealed and connected, forms two or more micro reaction tanks which may control temperatures independently, and the temperature controlling units are positioned in the reaction tanks. The utility model has the advantages of high integration level, quick analysis speed, less samples in need, flexible application and wide applicable range.