Method for detecting lithium concentration and lithium diagnostic kit

Enzymatic reaction continuous monitoring method of using lithium ion needed sensitized cyclohexanhexol -1(4) - phosphatase (CP) united to glyceraldehyde -3- phosphate dehydrogenase (GPD) is adopted in the invention. CP carries out reaction of enzymolysis for cyclohexanhexol 1- phosphoric acid to gen...

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1. Verfasser: ERZHONG WANG
Format: Patent
Sprache:eng
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Zusammenfassung:Enzymatic reaction continuous monitoring method of using lithium ion needed sensitized cyclohexanhexol -1(4) - phosphatase (CP) united to glyceraldehyde -3- phosphate dehydrogenase (GPD) is adopted in the invention. CP carries out reaction of enzymolysis for cyclohexanhexol 1- phosphoric acid to generate phosphate radical. Using action of united GPD deoxidizes coenzyme to reduced coenzyme so as to be able to measure upward velocity of absorbency of the reduced coenzyme at 340nm. Concentration of lithium can be calculated from the said upward speed of absorbency at 340nm. Features are: high specificity, and accurate test result. Using a pair of agent or Triple agent for kit, the invention can reduce intercross influence from each component. Through ultraviolet / visible light analytical apparatus, or semi-automatic/full automatic biochemical analysis apparatus the invention can obtain measured result quickly so that it is in low cost and is convenient to be popularized further.