Process for the solvent-based extraction of polyhydroxyalkanoates from biomass

Improved processes for extracting polyhydroxyalkanoate from a biomass containing the polyhydroxyalkanoate including the steps of: combining the biomass containing the polyhydroxyalkanoate with a solvent selected from lower chain ketones, and mixtures thereof to form a biomass liquor wherein the biom...

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Bibliographische Detailangaben
1. Verfasser:	NARASIMHAN KARUNAKARAN,CEARLEY ANGELLA C.,GIBSON MICHAEL S.,WELLING STANLEY J
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS BASED THEREON ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONSONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING ORGANIC MACROMOLECULAR COMPOUNDS PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL SEPARATION SPIRITS THEIR PREPARATION OR CHEMICAL WORKING-UP TRANSPORTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	Improved processes for extracting polyhydroxyalkanoate from a biomass containing the polyhydroxyalkanoate including the steps of: combining the biomass containing the polyhydroxyalkanoate with a solvent selected from lower chain ketones, and mixtures thereof to form a biomass liquor wherein the biomass liquor comprises less than about 25% water; mixing the biomass liquor for from about 10 to about 300 minutes at a temperature in the range of from about 70 DEG C to about 120 DEG C; separating the polyhydroxyalkanoate from the biomass liquor to form a PHA-enriched liquor, wherein the separating occurs at a temperature of at least about 40 DEG C; mixing the PHA-enriched liquor with water to form precipitated polyhydroxyalkanoate and an impure solvent liquor, wherein the water is mixed with the PHA-enriched liquor in the ratio of from at least least 3 parts water to one part polyhydroxyalkanoate; and recovering the precipitated polyhydroxyalkanoate from the impure solvent liquor. Optional treatment with oxidizing agents or mild surfactant or mild bleach is used to enhance color and odor.