Method for assaying ion calcium concentration and ion calcium diagnostic kit
The invention relates to a detection method and the regent box for the Ca2+ concentration. The NAD(P)H oxidase need to be reactivated by the Ca2+ when it reacts, so The NAD(P)H oxidase is to oxidize the reduced coenzyme (has the absorb wave in 340nm) to oxidized coenzyme (has no absorb wave in 340nm...
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Zusammenfassung: | The invention relates to a detection method and the regent box for the Ca2+ concentration. The NAD(P)H oxidase need to be reactivated by the Ca2+ when it reacts, so The NAD(P)H oxidase is to oxidize the reduced coenzyme (has the absorb wave in 340nm) to oxidized coenzyme (has no absorb wave in 340nm). So it can detect the degree of absorbency decreasing in 340nm of reduced coenzyme to get the Ca2+ density. The method has the high specialty and precise testing result. The diagnosing regent box is made to double regent to decrease the cross influence and keep the solution stable. The invention can get the result by the ultraviolet/visible light analyzer. |
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