Method and kit for pesticide analysis

A method for rapid detection of pesticide at ppt level using immunochemiluminescence is diclosed. The method comprises packing immobilized antibodies packed in a glass capillary column, passing a sample solution to be tested for pesticide content through said column so that a pesticide-antibody conj...

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Bibliographische Detailangaben
1. Verfasser:	THAKUR MUNNA SINGH,KARANTH NAIKANKATTE GANESH,KUMAR MYSORE ANANTHARAMAIAH,AMITA RANI BANGALORE ESHWAR,PASHA AKIMAL,KARANTH NITTIRU GOPALA KRISHNA
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	A method for rapid detection of pesticide at ppt level using immunochemiluminescence is diclosed. The method comprises packing immobilized antibodies packed in a glass capillary column, passing a sample solution to be tested for pesticide content through said column so that a pesticide-antibody conjugate is formed, removing said pesticide-antibody conjugate from the column and adding thereto, urea H202 followed by Luminol and K3Fe(CN)6, mixing said solution thoroughly until light is generated, and capturing the light generated by a CCD camera, the intensity of the light produced being directly proportional to the amount of pesticide content in said sample. The present invention also discloses a kit for carrying out the method of the present invention.