Method of preparing virus sample parlicle of human papillomavirus

This invention relates to the method of producing human papilloma virus sample particles; it belongs to biotechnology field. The procedures are showed as following steps: the gene of human papilloma virus late albumen said is cloned to bichi yeast secretion expression vector; the bichi yeast cell is reconstructed. Secretion expression L1 albumen is induced by the yeast cell and methanol, it is cultured upper heat removing and purified to get L1 albumen, then the albumen can auto assembling virus sample particles. The advantages are that 1) L1 can auto assembling VLPs. 2) the sample particle has similar structure to natural particles, it can auto assemble virus to form good uniformity virus sample virus. 3) The yeast cell can realizes large scale fermentation, L1 albumen is secreted to yeast upper heat removing, the purification technique is simple, cost is low and it is propitious to large scale generating.