Arthrodial cartilage proto micro-carrier

A proto-microcarrier for arthrodial cartilage is prepared from fresh joint cartilage through freeze drying, pulverizing, sieving adding trypsin to the particles, oscillating at 37 deg.C for 24-48 hr, washing with distilled water three times, adding TritonX-100, oscillating at 4 deg.C for 48-72 hr, f...

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1. Verfasser: SHIBI,ZHANG LU
Format: Patent
Sprache:eng
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Zusammenfassung:A proto-microcarrier for arthrodial cartilage is prepared from fresh joint cartilage through freeze drying, pulverizing, sieving adding trypsin to the particles, oscillating at 37 deg.C for 24-48 hr, washing with distilled water three times, adding TritonX-100, oscillating at 4 deg.C for 48-72 hr, flushing with distilled water for 48 hr, centrifugal separation of deposit, mixing with distilled water, freeze drying, UV irradiating, Co60 irradiation, digestion by trypsin, stirring, pulverizing and washing with distilled water three times.