Biocatalyst for producing d-lactic acid

It is intended to provide a process for highly producing D-lactic acid. It is also intended to provide a process for highly selectively producing D-lactic acid having a high optical purity with the formation of little organic acids as by-products. D-Lactic acid is produced by culturing a microorgani...

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1. Verfasser:	WADA MITSUFUMI,OIKAWA TOSHIHIRO,MOCHIZUKI DAISUKE,TOKUDA JUNKO,KAWASHIMA MIYUKI,ARAKI TADASHI,ABE REIKO,MIYAKE HITOKI,TAKAHASHI HITOSHI,SAWAI HIDEKI,MIMIZUKA TAKASHI,MORISHIGE TAKASHI,HIGASHI YOSUKE
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	It is intended to provide a process for highly producing D-lactic acid. It is also intended to provide a process for highly selectively producing D-lactic acid having a high optical purity with the formation of little organic acids as by-products. D-Lactic acid is produced by culturing a microorganism having inactivated or lowered pyruvate formate-lyase activity and elevated Escherichia coli-origin NADH-dependent D-lactic acid dehydrogenase (ldhA) activity, a microorganism having inactivated or lowered FAD-dependent D-lactic acid dehydrogenase activity, or a microorganism as described above having a TCA cycle, inactivated or lowered malic acid dehydrogenase activity and inactivated or lowered aspartic acid ammonia-lyase activity. The ldhA activity is elevated by ligating a gene encoding ldhA to a promoter of a gene controlling the expression of a protein, which participates in a glycolysis system, a nucleic acid biosynthesis system or an amino acid biosynthesis system, on genome.