Preparation method of recombinant baculoviral for constructing fowl vaccine

This invention relates to a recombinant rob preparation for preparing bird vaccine. The method as follows: (1) constructing the carrier with the CMV-IE promoter; (2) constructing the recombining carrier with the CMV-IE promoter and external source gene; (3) constructing the recombining rob virus, co...

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1. Verfasser:	ZHUANGWEI,PING LOU
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	This invention relates to a recombinant rob preparation for preparing bird vaccine. The method as follows: (1) constructing the carrier with the CMV-IE promoter; (2) constructing the recombining carrier with the CMV-IE promoter and external source gene; (3) constructing the recombining rob virus, collecting the virus particles and measuring the effect of the virus; (4) checking the expression of the external source gene of the recombining rob virus; wherein the step (3) includes: 1 the recombining carrier transforming the DH10Bac cell; 2distilling the recombining rob virus Bacmid DNA; 3 PCR expanding Bacmid DNA; 4 Bacmid DNA dyeing the Sf9 cell to obtain the recombining rob virus; 5 expanding the recombining rob virus; 6 speckle eroding test; 7 speckle eroding purification; 8 collecting the recombining rob virus particles; 9 measuring the recombining rob virus effect.