Translocating enzyme as a selection marker
The subject of the present invention is a selection system for microorganisms, which is based on the inactivation of an essential translocating enzyme and the curing of this inactivation by means of an indentically acting factor which is made available to the cells concerned by means of a vector. On...
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Patent |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | HINTZ MAREN,FREUDL ROLAND,FEESCHE JORG,BREVES ROLAND |
| description | The subject of the present invention is a selection system for microorganisms, which is based on the inactivation of an essential translocating enzyme and the curing of this inactivation by means of an indentically acting factor which is made available to the cells concerned by means of a vector. One important area of application for this system is includes processses for protein production by culturing cells of a microorganism strain that are characterized by this selection system, particularly such that the transgene of interest is located on the same vector performing the cure. Appropriate microorganisms, possible uses for genes of translocation enzymes and vectors are likewise presented, including in particular the use of the gene secA from gram-negative or gram-positive bacteria such as B. licheniformis. |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_CN1756835A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CN1756835A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_CN1756835A3</originalsourceid><addsrcrecordid>eNrjZNAKKUrMK87JT04sycxLV0jNq6rMTVVILFZIVChOzUlNLsnMz1PITSzKTi3iYWBNS8wpTuWF0twM8m6uIc4euqkF-fGpxQWJyal5qSXxzn6G5qZmFsamjsaEVQAAotMoYQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Translocating enzyme as a selection marker</title><source>esp@cenet</source><creator>HINTZ MAREN,FREUDL ROLAND,FEESCHE JORG,BREVES ROLAND</creator><creatorcontrib>HINTZ MAREN,FREUDL ROLAND,FEESCHE JORG,BREVES ROLAND</creatorcontrib><description>The subject of the present invention is a selection system for microorganisms, which is based on the inactivation of an essential translocating enzyme and the curing of this inactivation by means of an indentically acting factor which is made available to the cells concerned by means of a vector. One important area of application for this system is includes processses for protein production by culturing cells of a microorganism strain that are characterized by this selection system, particularly such that the transgene of interest is located on the same vector performing the cure. Appropriate microorganisms, possible uses for genes of translocation enzymes and vectors are likewise presented, including in particular the use of the gene secA from gram-negative or gram-positive bacteria such as B. licheniformis.</description><language>eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; ORGANIC CHEMISTRY ; PEPTIDES ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2006</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20060405&DB=EPODOC&CC=CN&NR=1756835A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76419</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20060405&DB=EPODOC&CC=CN&NR=1756835A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>HINTZ MAREN,FREUDL ROLAND,FEESCHE JORG,BREVES ROLAND</creatorcontrib><title>Translocating enzyme as a selection marker</title><description>The subject of the present invention is a selection system for microorganisms, which is based on the inactivation of an essential translocating enzyme and the curing of this inactivation by means of an indentically acting factor which is made available to the cells concerned by means of a vector. One important area of application for this system is includes processses for protein production by culturing cells of a microorganism strain that are characterized by this selection system, particularly such that the transgene of interest is located on the same vector performing the cure. Appropriate microorganisms, possible uses for genes of translocation enzymes and vectors are likewise presented, including in particular the use of the gene secA from gram-negative or gram-positive bacteria such as B. licheniformis.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2006</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZNAKKUrMK87JT04sycxLV0jNq6rMTVVILFZIVChOzUlNLsnMz1PITSzKTi3iYWBNS8wpTuWF0twM8m6uIc4euqkF-fGpxQWJyal5qSXxzn6G5qZmFsamjsaEVQAAotMoYQ</recordid><startdate>20060405</startdate><enddate>20060405</enddate><creator>HINTZ MAREN,FREUDL ROLAND,FEESCHE JORG,BREVES ROLAND</creator><scope>EVB</scope></search><sort><creationdate>20060405</creationdate><title>Translocating enzyme as a selection marker</title><author>HINTZ MAREN,FREUDL ROLAND,FEESCHE JORG,BREVES ROLAND</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN1756835A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng</language><creationdate>2006</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>HINTZ MAREN,FREUDL ROLAND,FEESCHE JORG,BREVES ROLAND</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>HINTZ MAREN,FREUDL ROLAND,FEESCHE JORG,BREVES ROLAND</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Translocating enzyme as a selection marker</title><date>2006-04-05</date><risdate>2006</risdate><abstract>The subject of the present invention is a selection system for microorganisms, which is based on the inactivation of an essential translocating enzyme and the curing of this inactivation by means of an indentically acting factor which is made available to the cells concerned by means of a vector. One important area of application for this system is includes processses for protein production by culturing cells of a microorganism strain that are characterized by this selection system, particularly such that the transgene of interest is located on the same vector performing the cure. Appropriate microorganisms, possible uses for genes of translocation enzymes and vectors are likewise presented, including in particular the use of the gene secA from gram-negative or gram-positive bacteria such as B. licheniformis.</abstract><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | eng |
| recordid | cdi_epo_espacenet_CN1756835A |
| source | esp@cenet |
| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Translocating enzyme as a selection marker |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T05%3A43%3A05IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=HINTZ%20MAREN,FREUDL%20ROLAND,FEESCHE%20JORG,BREVES%20ROLAND&rft.date=2006-04-05&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3ECN1756835A%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |