Translocating enzyme as a selection marker

The subject of the present invention is a selection system for microorganisms, which is based on the inactivation of an essential translocating enzyme and the curing of this inactivation by means of an indentically acting factor which is made available to the cells concerned by means of a vector. On...

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Bibliographische Detailangaben
1. Verfasser:	HINTZ MAREN,FREUDL ROLAND,FEESCHE JORG,BREVES ROLAND
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The subject of the present invention is a selection system for microorganisms, which is based on the inactivation of an essential translocating enzyme and the curing of this inactivation by means of an indentically acting factor which is made available to the cells concerned by means of a vector. One important area of application for this system is includes processses for protein production by culturing cells of a microorganism strain that are characterized by this selection system, particularly such that the transgene of interest is located on the same vector performing the cure. Appropriate microorganisms, possible uses for genes of translocation enzymes and vectors are likewise presented, including in particular the use of the gene secA from gram-negative or gram-positive bacteria such as B. licheniformis.