Gene sequence in enrichment protein of late stage embryo in third group of vinca rosea

The third group late embryo protein of a kind of Changchun flower and coding product amino acid sequence, it relates to a kind of new gene sequence. Its character lies in as follows, the length of gene is 829bp, it has the beginning code ATG at 127bp, it has the ending code TGA at 618bp, it has the accessional code polyA at 806bp. The sequence has no son, and has intact opening knowing code frame at 492bp, creating 163amino acid code. The nucleotide acid sequence and carrot sequence have the same source, which is up to 69%. The nucleotide sequence and chick bean group late cembryo protein have the same source, which is up to 51%.It includes six basis sequence, which has eleven amino acid residue. This invention also provides a kind of a way which can get the growing gene rapidly. First it uses measurable drought dealing condition, to make the bearable drought gene expression rich. Second according to the conduction library cDNA and detect sequence, in a shortest time it can get the growing gene relating to bearable drought. This kind of gene conduction on the real nucleus expression carrier particle pCMV, it can detect sequence using t3 and t7.Meanwhile,it can filter real nucleus biology expression directly. This invention departs the third group late cembryo protein growing gene from the plant cDNA, The gene has a good bearable drought ability. Meanwhile, it has other good effect when faced to terrible circumstance, such as cold and salty. The copy of the gene expands the gene source of bearable drought research. It can enhance bearable drought ability using gene project technique, and provides richer and better selecting gene for the excellent character.